Detailed information of TA system TA103435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4115284..4115834
• Replicon: chromosome
• Accession: NZ_CP028745
• Organism: Escherichia coli strain A30

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: AOY83_RS19495
• Protein ID: WP_001160975.1
• Coordinates: 4115284..4115598 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: AOY83_RS19500
• Protein ID: WP_000125566.1
• Coordinates: 4115601..4115834 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY83_RS19470 (4110959)	4110959..4112245	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
AOY83_RS19475 (4112245)	4112245..4113234	+	990	WP_044189225.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
AOY83_RS19480 (4113231)	4113231..4114052	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
AOY83_RS19485 (4114055)	4114055..4114432	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
AOY83_RS19490 (4114439)	4114439..4115287	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
AOY83_RS19495 (4115284)	4115284..4115598	-	315	WP_001160975.1	CcdB family protein	Toxin
AOY83_RS19500 (4115601)	4115601..4115834	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
AOY83_RS19505 (4115920)	4115920..4116399	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
AOY83_RS19510 (4116591)	4116591..4118453	-	1863	WP_000377191.1	glutathione-regulated potassium-efflux system protein KefC	-
AOY83_RS19515 (4118446)	4118446..4118976	-	531	WP_000600718.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
AOY83_RS19520 (4119087)	4119087..4120418	-	1332	WP_001183198.1	MFS transporter	-
AOY83_RS19525 (4120477)	4120477..4120764	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T103435 WP_001160975.1 NZ_CP028745:c4115598-4115284 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T103435 NZ_CP028745:c4115598-4115284 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT103435 WP_000125566.1 NZ_CP028745:c4115834-4115601 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT103435 NZ_CP028745:c4115834-4115601 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9