Detailed information of TA system TA103432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3701573..3702410
• Replicon: chromosome
• Accession: NZ_CP028745
• Organism: Escherichia coli strain A30

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A080JA52
• Locus tag: AOY83_RS17615
• Protein ID: WP_032170811.1
• Coordinates: 3701868..3702410 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A0A080JFU1
• Locus tag: AOY83_RS17610
• Protein ID: WP_001577859.1
• Coordinates: 3701573..3701884 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY83_RS17585 (3696593)	3696593..3697540	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
AOY83_RS17590 (3697562)	3697562..3699553	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AOY83_RS17595 (3699543)	3699543..3700157	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AOY83_RS17600 (3700157)	3700157..3700486	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AOY83_RS17605 (3700498)	3700498..3701388	+	891	WP_000971336.1	heme o synthase	-
AOY83_RS17610 (3701573)	3701573..3701884	+	312	WP_001577859.1	DUF1778 domain-containing protein	Antitoxin
AOY83_RS17615 (3701868)	3701868..3702410	+	543	WP_032170811.1	GNAT family N-acetyltransferase	Toxin
AOY83_RS17620 (3702466)	3702466..3703401	-	936	WP_089628795.1	tetratricopeptide repeat protein	-
AOY83_RS17625 (3703808)	3703808..3705172	+	1365	WP_001000957.1	MFS transporter	-
AOY83_RS17630 (3705300)	3705300..3705791	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
AOY83_RS17635 (3705959)	3705959..3706870	+	912	WP_240767380.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19835.05 Da
• Isoelectric Point: 8.3395

>T103432 WP_032170811.1 NZ_CP028745:3701868-3702410 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRTSLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T103432 NZ_CP028745:3701868-3702410 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCACATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAGCCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTGGGGGTGGCGCG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11400.08 Da
• Isoelectric Point: 10.5548

>AT103432 WP_001577859.1 NZ_CP028745:3701573-3701884 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNRLREAAQAVGLDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT103432 NZ_CP028745:3701573-3701884 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAGACTTCGTGAAGCTGCGCAAGCCGTTGGGTTAGATTTAAGCGCCTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGCGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCC
GAACCTGCTCAACCGACGCTCGCACTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A080JA52

Antitoxin

• AlphaFold DB: A0A080JFU1