Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2573337..2573975 Replicon chromosome
Accession NZ_CP028745
Organism Escherichia coli strain A30

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag AOY83_RS12260 Protein ID WP_240767170.1
Coordinates 2573799..2573975 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AOY83_RS12255 Protein ID WP_001270286.1
Coordinates 2573337..2573753 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY83_RS12235 (2568489) 2568489..2569430 - 942 WP_160438281.1 ABC transporter permease -
AOY83_RS12240 (2569431) 2569431..2570444 - 1014 WP_000220411.1 ABC transporter ATP-binding protein -
AOY83_RS12245 (2570462) 2570462..2571607 - 1146 WP_240767168.1 ABC transporter substrate-binding protein -
AOY83_RS12250 (2571852) 2571852..2573258 - 1407 WP_240767169.1 PLP-dependent aminotransferase family protein -
AOY83_RS12255 (2573337) 2573337..2573753 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
AOY83_RS12260 (2573799) 2573799..2573975 - 177 WP_240767170.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
AOY83_RS12265 (2574197) 2574197..2574427 + 231 WP_000494244.1 YncJ family protein -
AOY83_RS12270 (2574519) 2574519..2576480 - 1962 WP_240767171.1 23S rRNA 5-hydroxycytidine C2501 synthase -
AOY83_RS12275 (2576553) 2576553..2577089 - 537 WP_240767172.1 DNA-binding transcriptional regulator SutR -
AOY83_RS12280 (2577142) 2577142..2578353 + 1212 WP_274614133.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(8-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6689.73 Da        Isoelectric Point: 11.2298

>T103428 WP_240767170.1 NZ_CP028745:c2573975-2573799 [Escherichia coli]
VKQSESRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T103428 NZ_CP028745:c2573975-2573799 [Escherichia coli]
GTGAAACAAAGCGAGTCCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT103428 WP_001270286.1 NZ_CP028745:c2573753-2573337 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT103428 NZ_CP028745:c2573753-2573337 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References