Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 389824..390624 Replicon chromosome
Accession NZ_CP028745
Organism Escherichia coli strain A30

Toxin (Protein)


Gene name ataT Uniprot ID U9Y257
Locus tag AOY83_RS01795 Protein ID WP_000342447.1
Coordinates 390097..390624 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag AOY83_RS01790 Protein ID WP_001277108.1
Coordinates 389824..390090 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY83_RS01770 (385482) 385482..386150 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
AOY83_RS01775 (386143) 386143..387201 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
AOY83_RS01780 (387446) 387446..388300 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
AOY83_RS01785 (388571) 388571..389674 + 1104 WP_077758768.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
AOY83_RS01790 (389824) 389824..390090 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
AOY83_RS01795 (390097) 390097..390624 + 528 WP_000342447.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
AOY83_RS01800 (390621) 390621..391004 - 384 WP_240767635.1 aspartate 1-decarboxylase autocleavage activator PanM -
AOY83_RS01805 (391428) 391428..392537 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
AOY83_RS01810 (392585) 392585..393511 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
AOY83_RS01815 (393508) 393508..394785 + 1278 WP_000803782.1 branched chain amino acid ABC transporter permease LivM -
AOY83_RS01820 (394782) 394782..395549 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.63 Da        Isoelectric Point: 7.0284

>T103410 WP_000342447.1 NZ_CP028745:390097-390624 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDHSIQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T103410 NZ_CP028745:390097-390624 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTCACTCTTGGGCGTCTGGCAATTGATCATTCAATACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT103410 WP_001277108.1 NZ_CP028745:389824-390090 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT103410 NZ_CP028745:389824-390090 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAACATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LUM2

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References