Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 347009..347640 Replicon chromosome
Accession NZ_CP028745
Organism Escherichia coli strain A30

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag AOY83_RS01540 Protein ID WP_001259385.1
Coordinates 347009..347284 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AOY83_RS01545 Protein ID WP_000593555.1
Coordinates 347281..347640 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY83_RS01525 (342013) 342013..343080 + 1068 WP_000361491.1 HlyD family efflux transporter periplasmic adaptor subunit -
AOY83_RS01530 (343077) 343077..345812 + 2736 WP_175214811.1 ribosome-associated ATPase/putative transporter RbbA -
AOY83_RS01535 (345812) 345812..346936 + 1125 WP_001314210.1 ABC transporter permease -
AOY83_RS01540 (347009) 347009..347284 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
AOY83_RS01545 (347281) 347281..347640 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AOY83_RS01550 (347690) 347690..348550 - 861 WP_001131788.1 class II aldolase -
AOY83_RS01555 (348582) 348582..348851 - 270 WP_000933060.1 HPr family phosphocarrier protein -
AOY83_RS01560 (348841) 348841..350349 - 1509 WP_240767627.1 FGGY-family carbohydrate kinase -
AOY83_RS01565 (350342) 350342..351700 - 1359 WP_001308116.1 PTS galactitol transporter subunit IIC -
AOY83_RS01570 (351777) 351777..352058 - 282 WP_000084022.1 PTS sugar transporter subunit IIB -
AOY83_RS01575 (352055) 352055..352528 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 338153..347640 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T103409 WP_001259385.1 NZ_CP028745:347009-347284 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T103409 NZ_CP028745:347009-347284 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT103409 WP_000593555.1 NZ_CP028745:347281-347640 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT103409 NZ_CP028745:347281-347640 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References