Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4517242..4517828 Replicon chromosome
Accession NZ_CP028744
Organism Escherichia coli strain A31

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag AOY84_RS21905 Protein ID WP_042014162.1
Coordinates 4517598..4517828 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AOY84_RS21900 Protein ID WP_000593555.1
Coordinates 4517242..4517601 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY84_RS21875 (4513374) 4513374..4514318 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
AOY84_RS21880 (4514315) 4514315..4515148 + 834 WP_191633263.1 nickel ABC transporter permease subunit NikC -
AOY84_RS21885 (4515148) 4515148..4515912 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
AOY84_RS21890 (4515909) 4515909..4516715 + 807 WP_187185296.1 nickel import ATP-binding protein NikE -
AOY84_RS21895 (4516721) 4516721..4517122 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
AOY84_RS21900 (4517242) 4517242..4517601 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AOY84_RS21905 (4517598) 4517598..4517828 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
AOY84_RS21910 (4517932) 4517932..4519056 - 1125 WP_001314210.1 ABC transporter permease -
AOY84_RS21915 (4519056) 4519056..4521791 - 2736 WP_240754927.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T103408 WP_042014162.1 NZ_CP028744:c4517828-4517598 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T103408 NZ_CP028744:c4517828-4517598 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT103408 WP_000593555.1 NZ_CP028744:c4517601-4517242 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT103408 NZ_CP028744:c4517601-4517242 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References