Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3922871..3923525 Replicon chromosome
Accession NZ_CP028744
Organism Escherichia coli strain A31

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag AOY84_RS18905 Protein ID WP_000244772.1
Coordinates 3922871..3923278 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY84_RS18910 Protein ID WP_000354046.1
Coordinates 3923259..3923525 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY84_RS18885 (3918828) 3918828..3920561 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
AOY84_RS18890 (3920567) 3920567..3921277 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY84_RS18895 (3921302) 3921302..3922198 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AOY84_RS18900 (3922310) 3922310..3922831 + 522 WP_001055874.1 flavodoxin FldB -
AOY84_RS18905 (3922871) 3922871..3923278 - 408 WP_000244772.1 protein YgfX Toxin
AOY84_RS18910 (3923259) 3923259..3923525 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY84_RS18915 (3923768) 3923768..3924748 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AOY84_RS18920 (3924825) 3924825..3925484 - 660 WP_000250274.1 hemolysin III family protein -
AOY84_RS18925 (3925648) 3925648..3925959 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
AOY84_RS18930 (3926004) 3926004..3927437 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
AOY84_RS18935 (3927494) 3927494..3928237 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T103406 WP_000244772.1 NZ_CP028744:c3923278-3922871 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103406 NZ_CP028744:c3923278-3922871 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103406 WP_000354046.1 NZ_CP028744:c3923525-3923259 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103406 NZ_CP028744:c3923525-3923259 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References