Detailed information of TA system TA103404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3496677..3497302
• Replicon: chromosome
• Accession: NZ_CP028744
• Organism: Escherichia coli strain A31

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AOY84_RS16885
• Protein ID: WP_000911330.1
• Coordinates: 3496677..3497075 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: AOY84_RS16890
• Protein ID: WP_000450524.1
• Coordinates: 3497075..3497302 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY84_RS16865 (3492555)	3492555..3492755	+	201	WP_000383836.1	YpfN family protein	-
AOY84_RS16870 (3492865)	3492865..3493563	-	699	WP_000679823.1	esterase	-
AOY84_RS16875 (3493637)	3493637..3495652	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
AOY84_RS16880 (3495667)	3495667..3496530	-	864	WP_001267507.1	neutral zinc metallopeptidase	-
AOY84_RS16885 (3496677)	3496677..3497075	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
AOY84_RS16890 (3497075)	3497075..3497302	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
AOY84_RS16895 (3497456)	3497456..3498169	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
AOY84_RS16900 (3498382)	3498382..3499416	-	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
AOY84_RS16905 (3499433)	3499433..3500311	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AOY84_RS16910 (3500457)	3500457..3501029	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
AOY84_RS16915 (3501029)	3501029..3501499	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T103404 WP_000911330.1 NZ_CP028744:c3497075-3496677 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T103404 NZ_CP028744:c3497075-3496677 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT103404 WP_000450524.1 NZ_CP028744:c3497302-3497075 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT103404 NZ_CP028744:c3497302-3497075 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33