Detailed information of TA system TA103392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2330380..2331018
• Replicon: chromosome
• Accession: NZ_CP028744
• Organism: Escherichia coli strain A31

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: AOY84_RS11125
• Protein ID: WP_000813794.1
• Coordinates: 2330380..2330556 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AOY84_RS11130
• Protein ID: WP_001270286.1
• Coordinates: 2330602..2331018 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY84_RS11105 (2325999)	2325999..2327213	-	1215	WP_071609973.1	BenE family transporter YdcO	-
AOY84_RS11110 (2327266)	2327266..2327802	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
AOY84_RS11115 (2327875)	2327875..2329836	+	1962	WP_001442195.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AOY84_RS11120 (2329928)	2329928..2330158	-	231	WP_000494244.1	YncJ family protein	-
AOY84_RS11125 (2330380)	2330380..2330556	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AOY84_RS11130 (2330602)	2330602..2331018	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AOY84_RS11135 (2331097)	2331097..2332503	+	1407	WP_240755069.1	PLP-dependent aminotransferase family protein	-
AOY84_RS11140 (2332748)	2332748..2333893	+	1146	WP_000047455.1	ABC transporter substrate-binding protein	-
AOY84_RS11145 (2333911)	2333911..2334924	+	1014	WP_240755070.1	ABC transporter ATP-binding protein	-
AOY84_RS11150 (2334925)	2334925..2335866	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2324811..2325959	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T103392 WP_000813794.1 NZ_CP028744:2330380-2330556 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T103392 NZ_CP028744:2330380-2330556 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT103392 WP_001270286.1 NZ_CP028744:2330602-2331018 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT103392 NZ_CP028744:2330602-2331018 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB