Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1355553..1356171 Replicon chromosome
Accession NZ_CP028744
Organism Escherichia coli strain A31

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY84_RS06365 Protein ID WP_001291435.1
Coordinates 1355553..1355771 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY84_RS06370 Protein ID WP_000344800.1
Coordinates 1355797..1356171 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY84_RS06330 (1350842) 1350842..1351414 + 573 WP_000779826.1 YbaY family lipoprotein -
AOY84_RS06335 (1351445) 1351445..1351756 - 312 WP_000409911.1 MGMT family protein -
AOY84_RS06345 (1352135) 1352135..1352488 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY84_RS06350 (1352530) 1352530..1354080 - 1551 WP_001364591.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY84_RS06355 (1354244) 1354244..1354714 - 471 WP_000136192.1 YlaC family protein -
AOY84_RS06360 (1354830) 1354830..1355381 - 552 WP_000102564.1 maltose O-acetyltransferase -
AOY84_RS06365 (1355553) 1355553..1355771 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY84_RS06370 (1355797) 1355797..1356171 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY84_RS06375 (1356717) 1356717..1359866 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AOY84_RS06380 (1359889) 1359889..1361082 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103381 WP_001291435.1 NZ_CP028744:c1355771-1355553 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103381 NZ_CP028744:c1355771-1355553 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103381 WP_000344800.1 NZ_CP028744:c1356171-1355797 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103381 NZ_CP028744:c1356171-1355797 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References