Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3799992..3800646 Replicon chromosome
Accession NZ_CP028743
Organism Escherichia coli strain A32

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag AOY85_RS18255 Protein ID WP_000244781.1
Coordinates 3799992..3800399 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY85_RS18260 Protein ID WP_000354046.1
Coordinates 3800380..3800646 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY85_RS18235 (3795949) 3795949..3797682 - 1734 WP_000813193.1 single-stranded-DNA-specific exonuclease RecJ -
AOY85_RS18240 (3797688) 3797688..3798398 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY85_RS18245 (3798423) 3798423..3799319 - 897 WP_000806633.1 site-specific tyrosine recombinase XerD -
AOY85_RS18250 (3799431) 3799431..3799952 + 522 WP_001055874.1 flavodoxin FldB -
AOY85_RS18255 (3799992) 3799992..3800399 - 408 WP_000244781.1 protein YgfX Toxin
AOY85_RS18260 (3800380) 3800380..3800646 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY85_RS18265 (3800889) 3800889..3801869 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
AOY85_RS18270 (3801946) 3801946..3802605 - 660 WP_000250274.1 hemolysin III family protein -
AOY85_RS18275 (3802769) 3802769..3803080 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
AOY85_RS18280 (3803125) 3803125..3804558 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T103374 WP_000244781.1 NZ_CP028743:c3800399-3799992 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103374 NZ_CP028743:c3800399-3799992 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103374 WP_000354046.1 NZ_CP028743:c3800646-3800380 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103374 NZ_CP028743:c3800646-3800380 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References