Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2841990..2842580 Replicon chromosome
Accession NZ_CP028743
Organism Escherichia coli strain A32

Toxin (Protein)


Gene name pemK Uniprot ID V0VE57
Locus tag AOY85_RS13910 Protein ID WP_000373724.1
Coordinates 2841990..2842322 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag AOY85_RS13915 Protein ID WP_000288812.1
Coordinates 2842323..2842580 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY85_RS13890 (2837453) 2837453..2838715 + 1263 WP_001609141.1 integrase arm-type DNA-binding domain-containing protein -
AOY85_RS13895 (2838854) 2838854..2839312 - 459 WP_000526101.1 IS200/IS605-like element IS200C family transposase -
AOY85_RS13900 (2840264) 2840264..2841142 - 879 WP_000511773.1 integrase domain-containing protein -
AOY85_RS13905 (2841521) 2841521..2841943 + 423 WP_001164104.1 hypothetical protein -
AOY85_RS13910 (2841990) 2841990..2842322 - 333 WP_000373724.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AOY85_RS13915 (2842323) 2842323..2842580 - 258 WP_000288812.1 antitoxin Antitoxin
AOY85_RS13920 (2842678) 2842678..2842869 + 192 WP_077561361.1 hypothetical protein -
AOY85_RS13925 (2842928) 2842928..2843134 - 207 WP_000983730.1 helix-turn-helix transcriptional regulator -
AOY85_RS13930 (2843131) 2843131..2843598 - 468 WP_000201267.1 hypothetical protein -
AOY85_RS13935 (2843831) 2843831..2844967 - 1137 WP_000420971.1 ISAs1-like element ISEc1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2820337..2872510 52173
- inside IScluster/Tn - - 2838854..2844967 6113


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11724.57 Da        Isoelectric Point: 10.5834

>T103371 WP_000373724.1 NZ_CP028743:c2842322-2841990 [Escherichia coli]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

>T103371 NZ_CP028743:c2842322-2841990 [Escherichia coli]
ATGGACAGAGGGGAAATCTGGCTGGTTTCACTGGATCCGATTGCAGGTCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCCGTTGTTGTTCCCGTTACCAGCGGCGGAAACTTCGCCCGTG
CAGCCGGTTTTACCGTCTCACTGGACGGTGCGGGAACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGAGCCCGGAGCGGTAAGCGACTGGAACGTATACCCGATGCTGTCGTGAATGAAGTGCTGGCCCGACTGGAGGC
CATTCTGAGCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT103371 WP_000288812.1 NZ_CP028743:c2842580-2842323 [Escherichia coli]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT103371 NZ_CP028743:c2842580-2842323 [Escherichia coli]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCCCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGTTGTTGGCGCAGTGCGATCCGCACGCAGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VE57

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References