Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1367491..1368109 Replicon chromosome
Accession NZ_CP028743
Organism Escherichia coli strain A32

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY85_RS06625 Protein ID WP_001291435.1
Coordinates 1367491..1367709 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY85_RS06630 Protein ID WP_000344800.1
Coordinates 1367735..1368109 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY85_RS06590 (1362778) 1362778..1363350 + 573 WP_000779831.1 YbaY family lipoprotein -
AOY85_RS06595 (1363381) 1363381..1363692 - 312 WP_000409908.1 MGMT family protein -
AOY85_RS06605 (1364071) 1364071..1364424 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY85_RS06610 (1364466) 1364466..1366016 - 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY85_RS06615 (1366180) 1366180..1366650 - 471 WP_000136192.1 YlaC family protein -
AOY85_RS06620 (1366766) 1366766..1367317 - 552 WP_033549224.1 maltose O-acetyltransferase -
AOY85_RS06625 (1367491) 1367491..1367709 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY85_RS06630 (1367735) 1367735..1368109 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY85_RS06635 (1368654) 1368654..1371803 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
AOY85_RS06640 (1371826) 1371826..1373019 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103360 WP_001291435.1 NZ_CP028743:c1367709-1367491 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103360 NZ_CP028743:c1367709-1367491 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103360 WP_000344800.1 NZ_CP028743:c1368109-1367735 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103360 NZ_CP028743:c1368109-1367735 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References