Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 472998..473495 Replicon chromosome
Accession NZ_CP028743
Organism Escherichia coli strain A32

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AOY85_RS02170 Protein ID WP_001173343.1
Coordinates 473322..473495 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag AOY85_RS02165 Protein ID WP_001312331.1
Coordinates 472998..473294 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY85_RS02145 (469727) 469727..470446 - 720 WP_000611282.1 two-component system response regulator DcuR -
AOY85_RS02150 (470443) 470443..472074 - 1632 WP_001216466.1 sensor histidine kinase -
AOY85_RS02155 (472255) 472255..472485 + 231 WP_000371715.1 4Fe-4S mono-cluster protein YjdI -
AOY85_RS02160 (472497) 472497..472769 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
AOY85_RS02165 (472998) 472998..473294 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY85_RS02170 (473322) 473322..473495 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY85_RS02175 (473614) 473614..475131 - 1518 WP_000003806.1 lysine--tRNA ligase -
AOY85_RS02180 (475368) 475368..476825 - 1458 WP_000856825.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T103351 WP_001173343.1 NZ_CP028743:473322-473495 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T103351 NZ_CP028743:473322-473495 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT103351 WP_001312331.1 NZ_CP028743:472998-473294 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT103351 NZ_CP028743:472998-473294 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References