Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2910284..2910874 Replicon chromosome
Accession NZ_CP028742
Organism Escherichia coli strain A33

Toxin (Protein)


Gene name pemK Uniprot ID V0VE57
Locus tag AOY86_RS14140 Protein ID WP_000373724.1
Coordinates 2910284..2910616 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag AOY86_RS14145 Protein ID WP_000288812.1
Coordinates 2910617..2910874 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY86_RS14120 (2905785) 2905785..2907047 + 1263 WP_001609141.1 integrase arm-type DNA-binding domain-containing protein -
AOY86_RS14125 (2907186) 2907186..2907644 - 459 WP_000526101.1 IS200/IS605-like element IS200C family transposase -
AOY86_RS14130 (2908596) 2908596..2909436 - 841 Protein_2746 integrase domain-containing protein -
AOY86_RS14135 (2909815) 2909815..2910237 + 423 WP_001164104.1 hypothetical protein -
AOY86_RS14140 (2910284) 2910284..2910616 - 333 WP_000373724.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AOY86_RS14145 (2910617) 2910617..2910874 - 258 WP_000288812.1 antitoxin Antitoxin
AOY86_RS14150 (2910972) 2910972..2911163 + 192 WP_077561361.1 hypothetical protein -
AOY86_RS14155 (2911222) 2911222..2911428 - 207 WP_000983730.1 helix-turn-helix transcriptional regulator -
AOY86_RS14160 (2911425) 2911425..2911893 - 469 Protein_2752 hypothetical protein -
AOY86_RS14165 (2912127) 2912127..2913263 - 1137 WP_000420971.1 ISAs1-like element ISEc1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2893841..2940806 46965
- inside IScluster/Tn - - 2907186..2913263 6077


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11724.57 Da        Isoelectric Point: 10.5834

>T103344 WP_000373724.1 NZ_CP028742:c2910616-2910284 [Escherichia coli]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

>T103344 NZ_CP028742:c2910616-2910284 [Escherichia coli]
ATGGACAGAGGGGAAATCTGGCTGGTTTCACTGGATCCGATTGCAGGTCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCCGTTGTTGTTCCCGTTACCAGCGGCGGAAACTTCGCCCGTG
CAGCCGGTTTTACCGTCTCACTGGACGGTGCGGGAACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGAGCCCGGAGCGGTAAGCGACTGGAACGTATACCCGATGCTGTCGTGAATGAAGTGCTGGCCCGACTGGAGGC
CATTCTGAGCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT103344 WP_000288812.1 NZ_CP028742:c2910874-2910617 [Escherichia coli]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT103344 NZ_CP028742:c2910874-2910617 [Escherichia coli]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCCCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGTTGTTGGCGCAGTGCGATCCGCACGCAGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VE57

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References