Detailed information of TA system TA103333

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1951024..1951819
• Replicon: chromosome
• Accession: NZ_CP028742
• Organism: Escherichia coli strain A33

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: AOY86_RS09255
• Protein ID: WP_240768622.1
• Coordinates: 1951445..1951819 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P7R0L9
• Locus tag: AOY86_RS09250
• Protein ID: WP_001285482.1
• Coordinates: 1951024..1951398 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY86_RS09225 (1947904)	1947904..1948725	+	822	WP_001234582.1	DUF932 domain-containing protein	-
AOY86_RS09230 (1949062)	1949062..1949535	+	474	WP_001313575.1	antirestriction protein	-
AOY86_RS09235 (1949551)	1949551..1950027	+	477	WP_001313574.1	RadC family protein	-
AOY86_RS09240 (1950090)	1950090..1950311	+	222	WP_000692315.1	DUF987 domain-containing protein	-
AOY86_RS09245 (1950330)	1950330..1950974	+	645	WP_000086762.1	hypothetical protein	-
AOY86_RS09250 (1951024)	1951024..1951398	+	375	WP_001285482.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AOY86_RS09255 (1951445)	1951445..1951819	+	375	WP_240768622.1	TA system toxin CbtA family protein	Toxin
AOY86_RS09260 (1951816)	1951816..1952307	+	492	WP_000976859.1	DUF5983 family protein	-
AOY86_RS09265 (1952319)	1952319..1952516	+	198	WP_000839257.1	DUF957 domain-containing protein	-
AOY86_RS09270 (1952609)	1952609..1953451	+	843	WP_001280460.1	DUF4942 domain-containing protein	-
AOY86_RS09280 (1953922)	1953922..1954860	+	939	WP_000351277.1	glyoxylate/hydroxypyruvate reductase GhrA	-
AOY86_RS09285 (1954915)	1954915..1955652	+	738	WP_000283656.1	zinc-binding phosphatase	-
AOY86_RS09290 (1955676)	1955676..1956230	+	555	WP_001001913.1	molecular chaperone YcdY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13917.83 Da
• Isoelectric Point: 6.8517

>T103333 WP_240768622.1 NZ_CP028742:1951445-1951819 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLPRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T103333 NZ_CP028742:1951445-1951819 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCCC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATAACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13791.44 Da
• Isoelectric Point: 4.7511

>AT103333 WP_001285482.1 NZ_CP028742:1951024-1951398 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT103333 NZ_CP028742:1951024-1951398 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A0P7R0L9