Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1334622..1335240 Replicon chromosome
Accession NZ_CP028742
Organism Escherichia coli strain A33

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY86_RS06350 Protein ID WP_001291435.1
Coordinates 1334622..1334840 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY86_RS06355 Protein ID WP_000344800.1
Coordinates 1334866..1335240 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY86_RS06315 (1329910) 1329910..1330482 + 573 WP_000779831.1 YbaY family lipoprotein -
AOY86_RS06320 (1330513) 1330513..1330824 - 312 WP_000409908.1 MGMT family protein -
AOY86_RS06330 (1331203) 1331203..1331556 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY86_RS06335 (1331598) 1331598..1333148 - 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY86_RS06340 (1333312) 1333312..1333782 - 471 WP_000136192.1 YlaC family protein -
AOY86_RS06345 (1333898) 1333898..1334449 - 552 WP_000102539.1 maltose O-acetyltransferase -
AOY86_RS06350 (1334622) 1334622..1334840 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY86_RS06355 (1334866) 1334866..1335240 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY86_RS06360 (1335785) 1335785..1338934 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
AOY86_RS06365 (1338957) 1338957..1340150 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103332 WP_001291435.1 NZ_CP028742:c1334840-1334622 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103332 NZ_CP028742:c1334840-1334622 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103332 WP_000344800.1 NZ_CP028742:c1335240-1334866 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103332 NZ_CP028742:c1335240-1334866 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References