Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 470302..470799 Replicon chromosome
Accession NZ_CP028742
Organism Escherichia coli strain A33

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AOY86_RS02160 Protein ID WP_001173343.1
Coordinates 470626..470799 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag AOY86_RS02155 Protein ID WP_001312331.1
Coordinates 470302..470598 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY86_RS02135 (467031) 467031..467750 - 720 WP_000611288.1 two-component system response regulator DcuR -
AOY86_RS02140 (467747) 467747..469378 - 1632 WP_001216466.1 sensor histidine kinase -
AOY86_RS02145 (469559) 469559..469789 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
AOY86_RS02150 (469801) 469801..470073 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
AOY86_RS02155 (470302) 470302..470598 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY86_RS02160 (470626) 470626..470799 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY86_RS02165 (470918) 470918..472435 - 1518 WP_000003805.1 lysine--tRNA ligase -
AOY86_RS02170 (472672) 472672..474129 - 1458 WP_000856825.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 470302..479653 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T103325 WP_001173343.1 NZ_CP028742:470626-470799 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T103325 NZ_CP028742:470626-470799 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT103325 WP_001312331.1 NZ_CP028742:470302-470598 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT103325 NZ_CP028742:470302-470598 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References