Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4674951..4675582 Replicon chromosome
Accession NZ_CP028741
Organism Escherichia coli strain A34

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag AOY87_RS23090 Protein ID WP_001259388.1
Coordinates 4675307..4675582 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AOY87_RS23085 Protein ID WP_000593555.1
Coordinates 4674951..4675310 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY87_RS23060 (4671083) 4671083..4672027 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
AOY87_RS23065 (4672024) 4672024..4672857 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
AOY87_RS23070 (4672857) 4672857..4673621 + 765 WP_001136210.1 nickel import ATP-binding protein NikD -
AOY87_RS23075 (4673618) 4673618..4674424 + 807 WP_001586378.1 nickel import ATP-binding protein NikE -
AOY87_RS23080 (4674430) 4674430..4674831 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
AOY87_RS23085 (4674951) 4674951..4675310 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AOY87_RS23090 (4675307) 4675307..4675582 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
AOY87_RS23095 (4675655) 4675655..4676779 - 1125 WP_001216257.1 ABC transporter permease -
AOY87_RS23100 (4676779) 4676779..4679514 - 2736 WP_240765722.1 ribosome-associated ATPase/putative transporter RbbA -
AOY87_RS23105 (4679511) 4679511..4680578 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T103323 WP_001259388.1 NZ_CP028741:c4675582-4675307 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T103323 NZ_CP028741:c4675582-4675307 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT103323 WP_000593555.1 NZ_CP028741:c4675310-4674951 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT103323 NZ_CP028741:c4675310-4674951 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References