Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1183102..1183720 Replicon chromosome
Accession NZ_CP028741
Organism Escherichia coli strain A34

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY87_RS05555 Protein ID WP_001291435.1
Coordinates 1183102..1183320 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY87_RS05560 Protein ID WP_000344800.1
Coordinates 1183346..1183720 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY87_RS05520 (1178391) 1178391..1178963 + 573 WP_000779826.1 YbaY family lipoprotein -
AOY87_RS05525 (1178994) 1178994..1179305 - 312 WP_000409911.1 MGMT family protein -
AOY87_RS05535 (1179684) 1179684..1180037 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY87_RS05540 (1180079) 1180079..1181629 - 1551 WP_240765850.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY87_RS05545 (1181793) 1181793..1182263 - 471 WP_000136192.1 YlaC family protein -
AOY87_RS05550 (1182379) 1182379..1182930 - 552 WP_000102569.1 maltose O-acetyltransferase -
AOY87_RS05555 (1183102) 1183102..1183320 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY87_RS05560 (1183346) 1183346..1183720 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY87_RS05565 (1184266) 1184266..1187415 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AOY87_RS05570 (1187438) 1187438..1188631 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103307 WP_001291435.1 NZ_CP028741:c1183320-1183102 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103307 NZ_CP028741:c1183320-1183102 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103307 WP_000344800.1 NZ_CP028741:c1183720-1183346 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103307 NZ_CP028741:c1183720-1183346 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References