Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 387266..387763 Replicon chromosome
Accession NZ_CP028741
Organism Escherichia coli strain A34

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AOY87_RS01785 Protein ID WP_001173343.1
Coordinates 387590..387763 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag AOY87_RS01780 Protein ID WP_001425728.1
Coordinates 387266..387562 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY87_RS01760 (383997) 383997..384716 - 720 WP_000611288.1 two-component system response regulator DcuR -
AOY87_RS01765 (384713) 384713..386344 - 1632 WP_001216477.1 sensor histidine kinase -
AOY87_RS01770 (386525) 386525..386755 + 231 WP_016241784.1 4Fe-4S mono-cluster protein YjdI -
AOY87_RS01775 (386767) 386767..387039 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
AOY87_RS01780 (387266) 387266..387562 + 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY87_RS01785 (387590) 387590..387763 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY87_RS01790 (387882) 387882..389399 - 1518 WP_001295074.1 lysine--tRNA ligase -
AOY87_RS01795 (389636) 389636..391094 - 1459 Protein_341 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T103305 WP_001173343.1 NZ_CP028741:387590-387763 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T103305 NZ_CP028741:387590-387763 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT103305 WP_001425728.1 NZ_CP028741:387266-387562 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT103305 NZ_CP028741:387266-387562 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References