Detailed information of TA system TA103304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4448625..4449211
• Replicon: chromosome
• Accession: NZ_CP028740
• Organism: Escherichia coli strain A35

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: AOY88_RS21785
• Protein ID: WP_050877118.1
• Coordinates: 4448981..4449211 (-)
• Length: 77 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: AOY88_RS21780
• Protein ID: WP_000593555.1
• Coordinates: 4448625..4448984 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY88_RS21755 (4444757)	4444757..4445701	+	945	WP_000947068.1	nickel ABC transporter permease subunit NikB	-
AOY88_RS21760 (4445698)	4445698..4446531	+	834	WP_052892355.1	nickel ABC transporter permease subunit NikC	-
AOY88_RS21765 (4446531)	4446531..4447295	+	765	WP_001136236.1	nickel import ATP-binding protein NikD	-
AOY88_RS21770 (4447292)	4447292..4448098	+	807	WP_000173666.1	nickel import ATP-binding protein NikE	-
AOY88_RS21775 (4448104)	4448104..4448505	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
AOY88_RS21780 (4448625)	4448625..4448984	-	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AOY88_RS21785 (4448981)	4448981..4449211	-	231	WP_050877118.1	type II toxin-antitoxin system HicA family toxin	Toxin
AOY88_RS21790 (4449315)	4449315..4450439	-	1125	WP_001314210.1	ABC transporter permease	-
AOY88_RS21795 (4450439)	4450439..4453174	-	2736	WP_240766900.1	ribosome-associated ATPase/putative transporter RbbA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (15-65)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8403.79 Da
• Isoelectric Point: 8.5518

>T103304 WP_050877118.1 NZ_CP028740:c4449211-4448981 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGARCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Nucleotide

• Length: 231 bp

>T103304 NZ_CP028740:c4449211-4448981 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGGCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCGCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT103304 WP_000593555.1 NZ_CP028740:c4448984-4448625 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT103304 NZ_CP028740:c4448984-4448625 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6