Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3860604..3861258 Replicon chromosome
Accession NZ_CP028740
Organism Escherichia coli strain A35

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag AOY88_RS18810 Protein ID WP_000244777.1
Coordinates 3860604..3861011 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY88_RS18815 Protein ID WP_000354046.1
Coordinates 3860992..3861258 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY88_RS18790 (3856561) 3856561..3858294 - 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -
AOY88_RS18795 (3858300) 3858300..3859010 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY88_RS18800 (3859035) 3859035..3859931 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AOY88_RS18805 (3860043) 3860043..3860564 + 522 WP_001055874.1 flavodoxin FldB -
AOY88_RS18810 (3860604) 3860604..3861011 - 408 WP_000244777.1 protein YgfX Toxin
AOY88_RS18815 (3860992) 3860992..3861258 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY88_RS18820 (3861501) 3861501..3862481 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AOY88_RS18825 (3862734) 3862734..3863714 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
AOY88_RS18830 (3863837) 3863837..3864496 - 660 WP_000250274.1 hemolysin III family protein -
AOY88_RS18835 (3864660) 3864660..3864971 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3862734..3863714 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T103302 WP_000244777.1 NZ_CP028740:c3861011-3860604 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103302 NZ_CP028740:c3861011-3860604 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103302 WP_000354046.1 NZ_CP028740:c3861258-3860992 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103302 NZ_CP028740:c3861258-3860992 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References