Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3412751..3413376 Replicon chromosome
Accession NZ_CP028740
Organism Escherichia coli strain A35

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag AOY88_RS16645 Protein ID WP_000911329.1
Coordinates 3412751..3413149 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag AOY88_RS16650 Protein ID WP_000450524.1
Coordinates 3413149..3413376 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY88_RS16625 (3408629) 3408629..3408829 + 201 WP_000383836.1 YpfN family protein -
AOY88_RS16630 (3408939) 3408939..3409637 - 699 WP_000679823.1 esterase -
AOY88_RS16635 (3409711) 3409711..3411726 - 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
AOY88_RS16640 (3411741) 3411741..3412604 - 864 WP_001267498.1 neutral zinc metallopeptidase -
AOY88_RS16645 (3412751) 3412751..3413149 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
AOY88_RS16650 (3413149) 3413149..3413376 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
AOY88_RS16655 (3413531) 3413531..3414244 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
AOY88_RS16660 (3414457) 3414457..3415491 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
AOY88_RS16665 (3415508) 3415508..3416386 - 879 WP_001311023.1 4-hydroxy-tetrahydrodipicolinate synthase -
AOY88_RS16670 (3416532) 3416532..3417104 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
AOY88_RS16675 (3417104) 3417104..3417574 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T103300 WP_000911329.1 NZ_CP028740:c3413149-3412751 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T103300 NZ_CP028740:c3413149-3412751 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT103300 WP_000450524.1 NZ_CP028740:c3413376-3413149 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT103300 NZ_CP028740:c3413376-3413149 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References