Detailed information of TA system TA103289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2293801..2294439
• Replicon: chromosome
• Accession: NZ_CP028740
• Organism: Escherichia coli strain A35

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: AOY88_RS11270
• Protein ID: WP_000813794.1
• Coordinates: 2293801..2293977 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AOY88_RS11275
• Protein ID: WP_001270286.1
• Coordinates: 2294023..2294439 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY88_RS11250 (2289420)	2289420..2290634	-	1215	WP_072001413.1	BenE family transporter YdcO	-
AOY88_RS11255 (2290687)	2290687..2291223	+	537	WP_000429150.1	DNA-binding transcriptional regulator SutR	-
AOY88_RS11260 (2291296)	2291296..2293257	+	1962	WP_001446397.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AOY88_RS11265 (2293349)	2293349..2293579	-	231	WP_000494244.1	YncJ family protein	-
AOY88_RS11270 (2293801)	2293801..2293977	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AOY88_RS11275 (2294023)	2294023..2294439	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AOY88_RS11280 (2294518)	2294518..2295924	+	1407	WP_032218095.1	PLP-dependent aminotransferase family protein	-
AOY88_RS11285 (2296169)	2296169..2297314	+	1146	WP_032218096.1	ABC transporter substrate-binding protein	-
AOY88_RS11290 (2297332)	2297332..2298345	+	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
AOY88_RS11295 (2298346)	2298346..2299287	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2288233..2289342	1109

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T103289 WP_000813794.1 NZ_CP028740:2293801-2293977 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T103289 NZ_CP028740:2293801-2293977 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT103289 WP_001270286.1 NZ_CP028740:2294023-2294439 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT103289 NZ_CP028740:2294023-2294439 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB