Detailed information of TA system TA103279

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 422521..423018
• Replicon: chromosome
• Accession: NZ_CP028740
• Organism: Escherichia coli strain A35

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: AOY88_RS01950
• Protein ID: WP_001173343.1
• Coordinates: 422845..423018 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: AOY88_RS01945
• Protein ID: WP_096931177.1
• Coordinates: 422521..422817 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY88_RS01925 (419252)	419252..419971	-	720	WP_000611288.1	two-component system response regulator DcuR	-
AOY88_RS01930 (419968)	419968..421599	-	1632	WP_001216486.1	sensor histidine kinase	-
AOY88_RS01935 (421780)	421780..422010	+	231	WP_000371700.1	4Fe-4S mono-cluster protein YjdI	-
AOY88_RS01940 (422022)	422022..422294	+	273	WP_000405647.1	GNAT family N-acetyltransferase	-
AOY88_RS01945 (422521)	422521..422817	+	297	WP_096931177.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
AOY88_RS01950 (422845)	422845..423018	+	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
AOY88_RS01955 (423137)	423137..424654	-	1518	WP_001295074.1	lysine--tRNA ligase	-
AOY88_RS01960 (424891)	424891..426348	-	1458	WP_000856834.1	dipeptide/tripeptide permease DtpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T103279 WP_001173343.1 NZ_CP028740:422845-423018 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T103279 NZ_CP028740:422845-423018 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11440.95 Da
• Isoelectric Point: 4.1705

>AT103279 WP_096931177.1 NZ_CP028740:422521-422817 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLSENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT103279 NZ_CP028740:422521-422817 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTAGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAGTGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7U9QI41