Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4806418..4807049 Replicon chromosome
Accession NZ_CP028739
Organism Escherichia coli strain A36

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag AOY89_RS23470 Protein ID WP_001260301.1
Coordinates 4806774..4807049 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AOY89_RS23465 Protein ID WP_000593555.1
Coordinates 4806418..4806777 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY89_RS23435 (4801530) 4801530..4802003 + 474 WP_001161651.1 PTS sugar transporter subunit IIA -
AOY89_RS23440 (4802000) 4802000..4802281 + 282 WP_000084021.1 PTS sugar transporter subunit IIB -
AOY89_RS23445 (4802358) 4802358..4803716 + 1359 WP_001304938.1 PTS galactitol transporter subunit IIC -
AOY89_RS23450 (4803709) 4803709..4805217 + 1509 WP_001314209.1 FGGY-family carbohydrate kinase -
AOY89_RS23455 (4805207) 4805207..4805476 + 270 WP_000956442.1 HPr family phosphocarrier protein -
AOY89_RS23460 (4805508) 4805508..4806368 + 861 WP_021581124.1 class II aldolase -
AOY89_RS23465 (4806418) 4806418..4806777 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AOY89_RS23470 (4806774) 4806774..4807049 - 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
AOY89_RS23475 (4807122) 4807122..4808246 - 1125 WP_001296492.1 ABC transporter permease -
AOY89_RS23480 (4808246) 4808246..4810981 - 2736 WP_000149182.1 ribosome-associated ATPase/putative transporter RbbA -
AOY89_RS23485 (4810978) 4810978..4812045 - 1068 WP_000361454.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T103277 WP_001260301.1 NZ_CP028739:c4807049-4806774 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T103277 NZ_CP028739:c4807049-4806774 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT103277 WP_000593555.1 NZ_CP028739:c4806777-4806418 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT103277 NZ_CP028739:c4806777-4806418 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References