Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3890466..3891084 Replicon chromosome
Accession NZ_CP028739
Organism Escherichia coli strain A36

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY89_RS19035 Protein ID WP_001291435.1
Coordinates 3890866..3891084 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY89_RS19030 Protein ID WP_000344800.1
Coordinates 3890466..3890840 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY89_RS19020 (3885556) 3885556..3886749 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AOY89_RS19025 (3886772) 3886772..3889921 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
AOY89_RS19030 (3890466) 3890466..3890840 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY89_RS19035 (3890866) 3890866..3891084 + 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY89_RS19040 (3891257) 3891257..3891808 + 552 WP_000102539.1 maltose O-acetyltransferase -
AOY89_RS19045 (3891924) 3891924..3892394 + 471 WP_000136192.1 YlaC family protein -
AOY89_RS19050 (3892558) 3892558..3894108 + 1551 WP_001445575.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY89_RS19055 (3894150) 3894150..3894503 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY89_RS19065 (3894882) 3894882..3895193 + 312 WP_240745541.1 MGMT family protein -
AOY89_RS19070 (3895224) 3895224..3895796 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103273 WP_001291435.1 NZ_CP028739:3890866-3891084 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103273 NZ_CP028739:3890866-3891084 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103273 WP_000344800.1 NZ_CP028739:3890466-3890840 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103273 NZ_CP028739:3890466-3890840 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References