Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1255866..1256520 Replicon chromosome
Accession NZ_CP028739
Organism Escherichia coli strain A36

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag AOY89_RS06075 Protein ID WP_000244781.1
Coordinates 1256113..1256520 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY89_RS06070 Protein ID WP_000354046.1
Coordinates 1255866..1256132 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY89_RS06050 (1251954) 1251954..1253387 - 1434 WP_024262092.1 6-phospho-beta-glucosidase BglA -
AOY89_RS06055 (1253432) 1253432..1253743 + 312 WP_001182964.1 N(4)-acetylcytidine aminohydrolase -
AOY89_RS06060 (1253907) 1253907..1254566 + 660 WP_000250274.1 hemolysin III family protein -
AOY89_RS06065 (1254643) 1254643..1255623 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
AOY89_RS06070 (1255866) 1255866..1256132 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY89_RS06075 (1256113) 1256113..1256520 + 408 WP_000244781.1 protein YgfX Toxin
AOY89_RS06080 (1256560) 1256560..1257081 - 522 WP_001055874.1 flavodoxin FldB -
AOY89_RS06085 (1257193) 1257193..1258089 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AOY89_RS06090 (1258114) 1258114..1258824 + 711 WP_240745657.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY89_RS06095 (1258830) 1258830..1260563 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T103259 WP_000244781.1 NZ_CP028739:1256113-1256520 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103259 NZ_CP028739:1256113-1256520 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103259 WP_000354046.1 NZ_CP028739:1255866-1256132 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103259 NZ_CP028739:1255866-1256132 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References