Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 521268..521765 Replicon chromosome
Accession NZ_CP028739
Organism Escherichia coli strain A36

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AOY89_RS02485 Protein ID WP_001173343.1
Coordinates 521592..521765 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag AOY89_RS02480 Protein ID WP_001312331.1
Coordinates 521268..521564 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY89_RS02460 (517997) 517997..518716 - 720 WP_000611291.1 two-component system response regulator DcuR -
AOY89_RS02465 (518713) 518713..520344 - 1632 WP_001216466.1 sensor histidine kinase -
AOY89_RS02470 (520525) 520525..520755 + 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
AOY89_RS02475 (520767) 520767..521039 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
AOY89_RS02480 (521268) 521268..521564 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY89_RS02485 (521592) 521592..521765 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY89_RS02490 (521884) 521884..523401 - 1518 WP_000003807.1 lysine--tRNA ligase -
AOY89_RS02495 (523638) 523638..525095 - 1458 WP_000856849.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T103255 WP_001173343.1 NZ_CP028739:521592-521765 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T103255 NZ_CP028739:521592-521765 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT103255 WP_001312331.1 NZ_CP028739:521268-521564 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT103255 NZ_CP028739:521268-521564 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References