103251

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4324799..4325394 Replicon chromosome
Accession NZ_CP028738
Organism Escherichia coli strain A38

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag AOY91_RS21050 Protein ID WP_000239579.1
Coordinates 4324799..4325149 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag AOY91_RS21055 Protein ID WP_001223208.1
Coordinates 4325143..4325394 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY91_RS21030 (4320075) 4320075..4321097 - 1023 WP_001317446.1 ABC transporter permease -
AOY91_RS21035 (4321111) 4321111..4322613 - 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
AOY91_RS21040 (4322923) 4322923..4323879 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
AOY91_RS21045 (4324189) 4324189..4324719 + 531 WP_000055072.1 inorganic diphosphatase -
AOY91_RS21050 (4324799) 4324799..4325149 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
AOY91_RS21055 (4325143) 4325143..4325394 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
AOY91_RS21060 (4325607) 4325607..4325948 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
AOY91_RS21065 (4325951) 4325951..4329730 - 3780 WP_240727192.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T103251 WP_000239579.1 NZ_CP028738:c4325149-4324799 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T103251 NZ_CP028738:c4325149-4324799 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT103251 WP_001223208.1 NZ_CP028738:c4325394-4325143 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT103251 NZ_CP028738:c4325394-4325143 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References