Detailed information of TA system TA103248

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3623786..3624623
• Replicon: chromosome
• Accession: NZ_CP028738
• Organism: Escherichia coli strain A38

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: AOY91_RS17625
• Protein ID: WP_000227784.1
• Coordinates: 3624081..3624623 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AOY91_RS17620
• Protein ID: WP_001297137.1
• Coordinates: 3623786..3624097 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY91_RS17595 (3618806)	3618806..3619753	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
AOY91_RS17600 (3619775)	3619775..3621766	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AOY91_RS17605 (3621756)	3621756..3622370	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AOY91_RS17610 (3622370)	3622370..3622699	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AOY91_RS17615 (3622711)	3622711..3623601	+	891	WP_000971336.1	heme o synthase	-
AOY91_RS17620 (3623786)	3623786..3624097	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AOY91_RS17625 (3624081)	3624081..3624623	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
AOY91_RS17630 (3624679)	3624679..3625614	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
AOY91_RS17635 (3626022)	3626022..3627386	+	1365	WP_001000978.1	MFS transporter	-
AOY91_RS17640 (3627514)	3627514..3628005	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
AOY91_RS17645 (3628173)	3628173..3629084	+	912	WP_000705847.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T103248 WP_000227784.1 NZ_CP028738:3624081-3624623 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T103248 NZ_CP028738:3624081-3624623 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT103248 WP_001297137.1 NZ_CP028738:3623786-3624097 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT103248 NZ_CP028738:3623786-3624097 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3