Detailed information of TA system TA103245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2554520..2555158
• Replicon: chromosome
• Accession: NZ_CP028738
• Organism: Escherichia coli strain A38

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: AOY91_RS12410
• Protein ID: WP_000813794.1
• Coordinates: 2554982..2555158 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AOY91_RS12405
• Protein ID: WP_001270286.1
• Coordinates: 2554520..2554936 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY91_RS12385 (2549672)	2549672..2550613	-	942	WP_001251319.1	ABC transporter permease	-
AOY91_RS12390 (2550614)	2550614..2551627	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
AOY91_RS12395 (2551645)	2551645..2552790	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
AOY91_RS12400 (2553035)	2553035..2554441	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
AOY91_RS12405 (2554520)	2554520..2554936	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AOY91_RS12410 (2554982)	2554982..2555158	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AOY91_RS12415 (2555380)	2555380..2555610	+	231	WP_000494244.1	YncJ family protein	-
AOY91_RS12420 (2555702)	2555702..2557663	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AOY91_RS12425 (2557736)	2557736..2558272	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
AOY91_RS12430 (2558325)	2558325..2559539	+	1215	WP_001349372.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2559579..2560844	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T103245 WP_000813794.1 NZ_CP028738:c2555158-2554982 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T103245 NZ_CP028738:c2555158-2554982 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT103245 WP_001270286.1 NZ_CP028738:c2554936-2554520 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT103245 NZ_CP028738:c2554936-2554520 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB