103232

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1056051..1056634 Replicon chromosome
Accession NZ_CP028738
Organism Escherichia coli strain A38

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag AOY91_RS05090 Protein ID WP_000254745.1
Coordinates 1056299..1056634 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag AOY91_RS05085 Protein ID WP_000581937.1
Coordinates 1056051..1056299 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY91_RS05075 (1052390) 1052390..1053691 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
AOY91_RS05080 (1053739) 1053739..1055973 + 2235 WP_000226815.1 GTP pyrophosphokinase -
AOY91_RS05085 (1056051) 1056051..1056299 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
AOY91_RS05090 (1056299) 1056299..1056634 + 336 WP_000254745.1 endoribonuclease MazF Toxin
AOY91_RS05095 (1056705) 1056705..1057496 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
AOY91_RS05100 (1057724) 1057724..1059361 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
AOY91_RS05105 (1059449) 1059449..1060747 + 1299 WP_000036723.1 phosphopyruvate hydratase -
AOY91_RS05110 (1060803) 1060803..1061165 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T103232 WP_000254745.1 NZ_CP028738:1056299-1056634 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T103232 NZ_CP028738:1056299-1056634 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT103232 WP_000581937.1 NZ_CP028738:1056051-1056299 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT103232 NZ_CP028738:1056051-1056299 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LMB4

References