Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4268254..4268751 Replicon chromosome
Accession NZ_CP028737
Organism Escherichia coli strain A39

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AOY92_RS20515 Protein ID WP_001173343.1
Coordinates 4268254..4268427 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag AOY92_RS20520 Protein ID WP_000398619.1
Coordinates 4268455..4268751 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY92_RS20505 (4264924) 4264924..4266381 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
AOY92_RS20510 (4266618) 4266618..4268135 + 1518 WP_001295087.1 lysine--tRNA ligase -
AOY92_RS20515 (4268254) 4268254..4268427 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY92_RS20520 (4268455) 4268455..4268751 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY92_RS20525 (4268978) 4268978..4269250 - 273 WP_000405649.1 GNAT family N-acetyltransferase -
AOY92_RS20530 (4269262) 4269262..4269492 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
AOY92_RS20535 (4269673) 4269673..4271304 + 1632 WP_001216487.1 sensor histidine kinase -
AOY92_RS20540 (4271301) 4271301..4272020 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T103226 WP_001173343.1 NZ_CP028737:c4268427-4268254 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T103226 NZ_CP028737:c4268427-4268254 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT103226 WP_000398619.1 NZ_CP028737:c4268751-4268455 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT103226 NZ_CP028737:c4268751-4268455 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References