Detailed information of TA system TA103225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4170815..4171410
• Replicon: chromosome
• Accession: NZ_CP028737
• Organism: Escherichia coli strain A39

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: AOY92_RS20015
• Protein ID: WP_000239580.1
• Coordinates: 4170815..4171165 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: U9Y2K1
• Locus tag: AOY92_RS20020
• Protein ID: WP_001223208.1
• Coordinates: 4171159..4171410 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY92_RS19995 (4166091)	4166091..4167113	-	1023	WP_001296689.1	ABC transporter permease	-
AOY92_RS20000 (4167127)	4167127..4168629	-	1503	WP_000205791.1	sugar ABC transporter ATP-binding protein	-
AOY92_RS20005 (4168939)	4168939..4169895	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
AOY92_RS20010 (4170205)	4170205..4170735	+	531	WP_000055072.1	inorganic diphosphatase	-
AOY92_RS20015 (4170815)	4170815..4171165	-	351	WP_000239580.1	endoribonuclease toxin ChpB	Toxin
AOY92_RS20020 (4171159)	4171159..4171410	-	252	WP_001223208.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
AOY92_RS20025 (4171623)	4171623..4171964	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
AOY92_RS20030 (4171967)	4171967..4175746	-	3780	WP_240757893.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-115)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12539.50 Da
• Isoelectric Point: 5.6219

>T103225 WP_000239580.1 NZ_CP028737:c4171165-4170815 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEVLLRLQAVVE

Nucleotide

• Length: 351 bp

>T103225 NZ_CP028737:c4171165-4170815 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGT
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9271.58 Da
• Isoelectric Point: 4.4201

>AT103225 WP_001223208.1 NZ_CP028737:c4171410-4171159 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT103225 NZ_CP028737:c4171410-4171159 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LQ26