Detailed information of TA system TA103222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3473028..3473865
• Replicon: chromosome
• Accession: NZ_CP028737
• Organism: Escherichia coli strain A39

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: AOY92_RS16820
• Protein ID: WP_000227784.1
• Coordinates: 3473323..3473865 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AOY92_RS16815
• Protein ID: WP_001297137.1
• Coordinates: 3473028..3473339 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY92_RS16790 (3468048)	3468048..3468995	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
AOY92_RS16795 (3469017)	3469017..3471008	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AOY92_RS16800 (3470998)	3470998..3471612	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AOY92_RS16805 (3471612)	3471612..3471941	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AOY92_RS16810 (3471953)	3471953..3472843	+	891	WP_000971336.1	heme o synthase	-
AOY92_RS16815 (3473028)	3473028..3473339	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AOY92_RS16820 (3473323)	3473323..3473865	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
AOY92_RS16825 (3473921)	3473921..3474856	-	936	WP_001368479.1	tetratricopeptide repeat protein	-
AOY92_RS16830 (3475264)	3475264..3476628	+	1365	WP_240757830.1	MFS transporter	-
AOY92_RS16835 (3476756)	3476756..3477247	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
AOY92_RS16840 (3477415)	3477415..3478326	+	912	WP_105475349.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T103222 WP_000227784.1 NZ_CP028737:3473323-3473865 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T103222 NZ_CP028737:3473323-3473865 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT103222 WP_001297137.1 NZ_CP028737:3473028-3473339 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT103222 NZ_CP028737:3473028-3473339 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3