Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3438947..3439565 Replicon chromosome
Accession NZ_CP028737
Organism Escherichia coli strain A39

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY92_RS16650 Protein ID WP_001291435.1
Coordinates 3439347..3439565 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY92_RS16645 Protein ID WP_000344800.1
Coordinates 3438947..3439321 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY92_RS16635 (3434036) 3434036..3435229 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AOY92_RS16640 (3435252) 3435252..3438401 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AOY92_RS16645 (3438947) 3438947..3439321 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY92_RS16650 (3439347) 3439347..3439565 + 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY92_RS16655 (3439737) 3439737..3440288 + 552 WP_000102564.1 maltose O-acetyltransferase -
AOY92_RS16660 (3440404) 3440404..3440874 + 471 WP_000136192.1 YlaC family protein -
AOY92_RS16665 (3441038) 3441038..3442588 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY92_RS16670 (3442630) 3442630..3442983 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY92_RS16680 (3443362) 3443362..3443673 + 312 WP_000409911.1 MGMT family protein -
AOY92_RS16685 (3443704) 3443704..3444276 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103221 WP_001291435.1 NZ_CP028737:3439347-3439565 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103221 NZ_CP028737:3439347-3439565 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103221 WP_000344800.1 NZ_CP028737:3438947-3439321 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103221 NZ_CP028737:3438947-3439321 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References