Detailed information of TA system TA103220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2431376..2432014
• Replicon: chromosome
• Accession: NZ_CP028737
• Organism: Escherichia coli strain A39

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: AOY92_RS11790
• Protein ID: WP_000813794.1
• Coordinates: 2431838..2432014 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AOY92_RS11785
• Protein ID: WP_001270286.1
• Coordinates: 2431376..2431792 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY92_RS11765 (2426528)	2426528..2427469	-	942	WP_001251319.1	ABC transporter permease	-
AOY92_RS11770 (2427470)	2427470..2428483	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
AOY92_RS11775 (2428501)	2428501..2429646	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
AOY92_RS11780 (2429888)	2429888..2431297	-	1410	WP_000760591.1	PLP-dependent aminotransferase family protein	-
AOY92_RS11785 (2431376)	2431376..2431792	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AOY92_RS11790 (2431838)	2431838..2432014	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AOY92_RS11795 (2432236)	2432236..2432466	+	231	WP_000494244.1	YncJ family protein	-
AOY92_RS11800 (2432558)	2432558..2434519	-	1962	WP_001442195.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AOY92_RS11805 (2434592)	2434592..2435128	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
AOY92_RS11810 (2435181)	2435181..2436395	+	1215	WP_071830296.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2436435..2437583	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T103220 WP_000813794.1 NZ_CP028737:c2432014-2431838 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T103220 NZ_CP028737:c2432014-2431838 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT103220 WP_001270286.1 NZ_CP028737:c2431792-2431376 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT103220 NZ_CP028737:c2431792-2431376 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB