Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 317442..318073 Replicon chromosome
Accession NZ_CP028737
Organism Escherichia coli strain A39

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag AOY92_RS01440 Protein ID WP_001259385.1
Coordinates 317442..317717 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AOY92_RS01445 Protein ID WP_000593555.1
Coordinates 317714..318073 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY92_RS01425 (312460) 312460..313527 + 1068 WP_021565451.1 HlyD family secretion protein -
AOY92_RS01430 (313524) 313524..316259 + 2736 WP_021565452.1 ribosome-associated ATPase/putative transporter RbbA -
AOY92_RS01435 (316259) 316259..317383 + 1125 WP_021565453.1 ABC transporter permease -
AOY92_RS01440 (317442) 317442..317717 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
AOY92_RS01445 (317714) 317714..318073 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AOY92_RS01450 (318123) 318123..318983 - 861 WP_240757959.1 class II aldolase -
AOY92_RS01455 (319015) 319015..319284 - 270 WP_000933061.1 HPr family phosphocarrier protein -
AOY92_RS01460 (319274) 319274..320782 - 1509 WP_021565454.1 FGGY-family carbohydrate kinase -
AOY92_RS01465 (320775) 320775..322133 - 1359 WP_021565455.1 PTS galactitol transporter subunit IIC -
AOY92_RS01470 (322210) 322210..322491 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
AOY92_RS01475 (322488) 322488..322961 - 474 WP_001161650.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T103205 WP_001259385.1 NZ_CP028737:317442-317717 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T103205 NZ_CP028737:317442-317717 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT103205 WP_000593555.1 NZ_CP028737:317714-318073 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT103205 NZ_CP028737:317714-318073 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References