103201

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4065681..4066335 Replicon chromosome
Accession NZ_CP028735
Organism Escherichia coli strain A41

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag AOY94_RS19765 Protein ID WP_000244772.1
Coordinates 4065681..4066088 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY94_RS19770 Protein ID WP_000354046.1
Coordinates 4066069..4066335 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY94_RS19745 (4061638) 4061638..4063371 - 1734 WP_240764390.1 single-stranded-DNA-specific exonuclease RecJ -
AOY94_RS19750 (4063377) 4063377..4064087 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY94_RS19755 (4064112) 4064112..4065008 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AOY94_RS19760 (4065120) 4065120..4065641 + 522 WP_001055874.1 flavodoxin FldB -
AOY94_RS19765 (4065681) 4065681..4066088 - 408 WP_000244772.1 protein YgfX Toxin
AOY94_RS19770 (4066069) 4066069..4066335 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY94_RS19775 (4066578) 4066578..4067558 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AOY94_RS19780 (4067930) 4067930..4068910 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
AOY94_RS19785 (4069033) 4069033..4069692 - 660 WP_000250274.1 hemolysin III family protein -
AOY94_RS19790 (4069856) 4069856..4070167 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4067930..4068910 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T103201 WP_000244772.1 NZ_CP028735:c4066088-4065681 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103201 NZ_CP028735:c4066088-4065681 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103201 WP_000354046.1 NZ_CP028735:c4066335-4066069 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103201 NZ_CP028735:c4066335-4066069 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QD57

References