Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1430644..1431262 Replicon chromosome
Accession NZ_CP028735
Organism Escherichia coli strain A41

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY94_RS06800 Protein ID WP_001291435.1
Coordinates 1430644..1430862 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY94_RS06805 Protein ID WP_000344800.1
Coordinates 1430888..1431262 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY94_RS06765 (1425934) 1425934..1426506 + 573 WP_000779831.1 YbaY family lipoprotein -
AOY94_RS06770 (1426537) 1426537..1426848 - 312 WP_000409911.1 MGMT family protein -
AOY94_RS06780 (1427227) 1427227..1427580 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY94_RS06785 (1427622) 1427622..1429172 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY94_RS06790 (1429336) 1429336..1429806 - 471 WP_000136192.1 YlaC family protein -
AOY94_RS06795 (1429922) 1429922..1430473 - 552 WP_032331097.1 maltose O-acetyltransferase -
AOY94_RS06800 (1430644) 1430644..1430862 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY94_RS06805 (1430888) 1430888..1431262 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY94_RS06810 (1431808) 1431808..1434957 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AOY94_RS06815 (1434980) 1434980..1436173 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103184 WP_001291435.1 NZ_CP028735:c1430862-1430644 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103184 NZ_CP028735:c1430862-1430644 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103184 WP_000344800.1 NZ_CP028735:c1431262-1430888 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103184 NZ_CP028735:c1431262-1430888 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References