Detailed information of TA system TA103183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1396349..1397186
• Replicon: chromosome
• Accession: NZ_CP028735
• Organism: Escherichia coli strain A41

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: AOY94_RS06630
• Protein ID: WP_000227784.1
• Coordinates: 1396349..1396891 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AOY94_RS06635
• Protein ID: WP_001297137.1
• Coordinates: 1396875..1397186 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY94_RS06610 (1391888)	1391888..1392799	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
AOY94_RS06615 (1392967)	1392967..1393458	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
AOY94_RS06620 (1393586)	1393586..1394950	-	1365	WP_001000955.1	MFS transporter	-
AOY94_RS06625 (1395358)	1395358..1396293	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
AOY94_RS06630 (1396349)	1396349..1396891	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
AOY94_RS06635 (1396875)	1396875..1397186	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AOY94_RS06640 (1397371)	1397371..1398261	-	891	WP_000971336.1	heme o synthase	-
AOY94_RS06645 (1398273)	1398273..1398602	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AOY94_RS06650 (1398602)	1398602..1399216	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AOY94_RS06655 (1399206)	1399206..1401197	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AOY94_RS06660 (1401219)	1401219..1402166	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T103183 WP_000227784.1 NZ_CP028735:c1396891-1396349 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T103183 NZ_CP028735:c1396891-1396349 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT103183 WP_001297137.1 NZ_CP028735:c1397186-1396875 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT103183 NZ_CP028735:c1397186-1396875 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3