Detailed information of TA system TA103179

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 536288..537078
• Replicon: chromosome
• Accession: NZ_CP028735
• Organism: Escherichia coli strain A41

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: F4VIU3
• Locus tag: AOY94_RS02535
• Protein ID: WP_000854832.1
• Coordinates: 536288..536626 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AOY94_RS02540
• Protein ID: WP_024186353.1
• Coordinates: 536716..537078 (-)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY94_RS02510 (531601)	531601..532659	+	1059	WP_021580234.1	site-specific integrase	-
AOY94_RS02515 (533052)	533052..534587	-	1536	WP_240764488.1	EAL domain-containing protein	-
AOY94_RS02520 (534658)	534658..535503	-	846	WP_240764489.1	DUF4942 domain-containing protein	-
AOY94_RS02525 (535588)	535588..535785	-	198	WP_000839273.1	DUF957 domain-containing protein	-
AOY94_RS02530 (535803)	535803..536291	-	489	WP_000777669.1	DUF5983 family protein	-
AOY94_RS02535 (536288)	536288..536626	-	339	WP_000854832.1	TA system toxin CbtA family protein	Toxin
AOY94_RS02540 (536716)	536716..537078	-	363	WP_024186353.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AOY94_RS02545 (537157)	537157..537378	-	222	WP_000692305.1	DUF987 domain-containing protein	-
AOY94_RS02550 (537447)	537447..537923	-	477	WP_001186726.1	RadC family protein	-
AOY94_RS02555 (537939)	537939..538424	-	486	WP_240764490.1	antirestriction protein	-
AOY94_RS02560 (538515)	538515..539333	-	819	WP_240764491.1	DUF932 domain-containing protein	-
AOY94_RS02565 (539424)	539424..539657	-	234	WP_001117566.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	espX5 / papH / papC / papD	363144..594118	230974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (5-99)

Antitoxin

• Antitoxin CbeA (16-115)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12734.56 Da
• Isoelectric Point: 9.6552

>T103179 WP_000854832.1 NZ_CP028735:c536626-536288 [Escherichia coli]
MKTLPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAFTRSQLINSID
ILRARRATGLMTRSNYSTVNNITRGKHSEAKR

Nucleotide

• Length: 339 bp

>T103179 NZ_CP028735:c536626-536288 [Escherichia coli]
ATGAAAACATTACCCGTCACCATCTGGCAGACACTGCTCACCCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGA
CACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGTATTTCACTGTGCGATGCGGTGAATTTTCTCG
TGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTCAGCGCCTTTACCCGCTCTCAGTTAATAAACAGTATTGAT
ATCCTCCGGGCACGCAGGGCAACCGGCCTGATGACCCGCAGCAACTACAGCACGGTGAACAATATCACCCGGGGTAAACA
CAGCGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13341.18 Da
• Isoelectric Point: 6.0537

>AT103179 WP_024186353.1 NZ_CP028735:c537078-536716 [Escherichia coli]
VSDTLPGTTHPDNNCPWWGLPCTVMPCFGARLVQEGNRLHYLTDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGEL
SPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 363 bp

>AT103179 NZ_CP028735:c537078-536716 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACAACAACTGCCCCTGGTGGGGGCTACCCTGCACCGTGATGCCCTG
TTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTACAGACCGCGCCGGTATCAGAGGCCGGTTCAGCG
ATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGTGAACTC
AGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACTTGCGAAGCCGATACCCTGGGCTCCTGTGGTTA
CGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAACGTAA

Structures

Toxin

• AlphaFold DB: F4VIU3