Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3446930..3447548 Replicon chromosome
Accession NZ_CP028734
Organism Escherichia coli strain A42

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY95_RS16800 Protein ID WP_001291435.1
Coordinates 3447330..3447548 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY95_RS16795 Protein ID WP_000344800.1
Coordinates 3446930..3447304 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY95_RS16785 (3442020) 3442020..3443213 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AOY95_RS16790 (3443236) 3443236..3446385 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
AOY95_RS16795 (3446930) 3446930..3447304 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY95_RS16800 (3447330) 3447330..3447548 + 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY95_RS16805 (3447721) 3447721..3448272 + 552 WP_000102539.1 maltose O-acetyltransferase -
AOY95_RS16810 (3448388) 3448388..3448858 + 471 WP_000136192.1 YlaC family protein -
AOY95_RS16815 (3449022) 3449022..3450573 + 1552 Protein_3302 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY95_RS16820 (3450615) 3450615..3450968 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY95_RS16830 (3451347) 3451347..3451658 + 312 WP_000409908.1 MGMT family protein -
AOY95_RS16835 (3451689) 3451689..3452261 - 573 WP_000779841.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103168 WP_001291435.1 NZ_CP028734:3447330-3447548 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103168 NZ_CP028734:3447330-3447548 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103168 WP_000344800.1 NZ_CP028734:3446930-3447304 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103168 NZ_CP028734:3446930-3447304 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References