Detailed information of TA system TA103143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3580433..3581270
• Replicon: chromosome
• Accession: NZ_CP028733
• Organism: Escherichia coli strain H2

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A0F5GET7
• Locus tag: AOY59_RS17465
• Protein ID: WP_000227783.1
• Coordinates: 3580728..3581270 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AOY59_RS17460
• Protein ID: WP_001297137.1
• Coordinates: 3580433..3580744 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY59_RS17435 (3575453)	3575453..3576400	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
AOY59_RS17440 (3576422)	3576422..3578413	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AOY59_RS17445 (3578403)	3578403..3579017	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AOY59_RS17450 (3579017)	3579017..3579346	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AOY59_RS17455 (3579358)	3579358..3580248	+	891	WP_000971336.1	heme o synthase	-
AOY59_RS17460 (3580433)	3580433..3580744	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AOY59_RS17465 (3580728)	3580728..3581270	+	543	WP_000227783.1	GNAT family N-acetyltransferase	Toxin
AOY59_RS17470 (3581326)	3581326..3582261	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
AOY59_RS17475 (3582669)	3582669..3584033	+	1365	WP_001000978.1	MFS transporter	-
AOY59_RS17480 (3584161)	3584161..3584652	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
AOY59_RS17485 (3584820)	3584820..3585731	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19776.96 Da
• Isoelectric Point: 8.0330

>T103143 WP_000227783.1 NZ_CP028733:3580728-3581270 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T103143 NZ_CP028733:3580728-3581270 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCAGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT103143 WP_001297137.1 NZ_CP028733:3580433-3580744 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT103143 NZ_CP028733:3580433-3580744 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A0F5GET7

Antitoxin

• AlphaFold DB: A0A0E0Y6B3