Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3546334..3546952 Replicon chromosome
Accession NZ_CP028733
Organism Escherichia coli strain H2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY59_RS17295 Protein ID WP_001291435.1
Coordinates 3546734..3546952 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY59_RS17290 Protein ID WP_000344800.1
Coordinates 3546334..3546708 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY59_RS17280 (3541423) 3541423..3542616 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AOY59_RS17285 (3542639) 3542639..3545788 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AOY59_RS17290 (3546334) 3546334..3546708 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY59_RS17295 (3546734) 3546734..3546952 + 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY59_RS17300 (3547124) 3547124..3547675 + 552 WP_000102564.1 maltose O-acetyltransferase -
AOY59_RS17305 (3547791) 3547791..3548261 + 471 WP_000136192.1 YlaC family protein -
AOY59_RS17310 (3548425) 3548425..3549996 + 1572 WP_096948927.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY59_RS17315 (3550038) 3550038..3550391 - 354 WP_000878141.1 DUF1428 family protein -
AOY59_RS17325 (3550770) 3550770..3551081 + 312 WP_000409911.1 MGMT family protein -
AOY59_RS17330 (3551112) 3551112..3551684 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103142 WP_001291435.1 NZ_CP028733:3546734-3546952 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103142 NZ_CP028733:3546734-3546952 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103142 WP_000344800.1 NZ_CP028733:3546334-3546708 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103142 NZ_CP028733:3546334-3546708 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References