Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1394330..1394955 Replicon chromosome
Accession NZ_CP028733
Organism Escherichia coli strain H2

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag AOY59_RS06755 Protein ID WP_000911329.1
Coordinates 1394557..1394955 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag AOY59_RS06750 Protein ID WP_000450524.1
Coordinates 1394330..1394557 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY59_RS06725 (1390132) 1390132..1390602 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
AOY59_RS06730 (1390602) 1390602..1391174 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
AOY59_RS06735 (1391320) 1391320..1392198 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
AOY59_RS06740 (1392215) 1392215..1393249 + 1035 WP_001355860.1 outer membrane protein assembly factor BamC -
AOY59_RS06745 (1393462) 1393462..1394175 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
AOY59_RS06750 (1394330) 1394330..1394557 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
AOY59_RS06755 (1394557) 1394557..1394955 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
AOY59_RS06760 (1395102) 1395102..1395965 + 864 WP_001267498.1 neutral zinc metallopeptidase -
AOY59_RS06765 (1395980) 1395980..1397995 + 2016 WP_000829393.1 tRNA cytosine(34) acetyltransferase TmcA -
AOY59_RS06770 (1398069) 1398069..1398767 + 699 WP_000679812.1 esterase -
AOY59_RS06775 (1398877) 1398877..1399077 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T103131 WP_000911329.1 NZ_CP028733:1394557-1394955 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T103131 NZ_CP028733:1394557-1394955 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT103131 WP_000450524.1 NZ_CP028733:1394330-1394557 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT103131 NZ_CP028733:1394330-1394557 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References