Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 913156..913810 Replicon chromosome
Accession NZ_CP028733
Organism Escherichia coli strain H2

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag AOY59_RS04480 Protein ID WP_000244772.1
Coordinates 913403..913810 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY59_RS04475 Protein ID WP_000354046.1
Coordinates 913156..913422 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY59_RS04450 (908444) 908444..909187 + 744 WP_001436959.1 SDR family oxidoreductase -
AOY59_RS04455 (909244) 909244..910677 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
AOY59_RS04460 (910722) 910722..911033 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
AOY59_RS04465 (911197) 911197..911856 + 660 WP_000250274.1 hemolysin III family protein -
AOY59_RS04470 (911933) 911933..912913 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AOY59_RS04475 (913156) 913156..913422 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY59_RS04480 (913403) 913403..913810 + 408 WP_000244772.1 protein YgfX Toxin
AOY59_RS04485 (913850) 913850..914371 - 522 WP_001055874.1 flavodoxin FldB -
AOY59_RS04490 (914483) 914483..915379 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AOY59_RS04495 (915404) 915404..916114 + 711 WP_096948976.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY59_RS04500 (916120) 916120..917853 + 1734 WP_096948977.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T103129 WP_000244772.1 NZ_CP028733:913403-913810 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103129 NZ_CP028733:913403-913810 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103129 WP_000354046.1 NZ_CP028733:913156-913422 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103129 NZ_CP028733:913156-913422 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References