Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 4067396..4068197 | Replicon | chromosome |
| Accession | NZ_CP028732 | ||
| Organism | Escherichia coli strain H3 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | D3GUT5 |
| Locus tag | AOY60_RS19750 | Protein ID | WP_001094430.1 |
| Coordinates | 4067820..4068197 (+) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | D3GUT4 |
| Locus tag | AOY60_RS19745 | Protein ID | WP_001285620.1 |
| Coordinates | 4067396..4067773 (+) | Length | 126 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AOY60_RS19710 (4063312) | 4063312..4063992 | + | 681 | WP_001278652.1 | WYL domain-containing protein | - |
| AOY60_RS19715 (4064140) | 4064140..4064817 | + | 678 | WP_001097302.1 | hypothetical protein | - |
| AOY60_RS19720 (4064823) | 4064823..4065056 | + | 234 | WP_001278293.1 | DUF905 family protein | - |
| AOY60_RS19725 (4065146) | 4065146..4065964 | + | 819 | WP_001175167.1 | DUF932 domain-containing protein | - |
| AOY60_RS19730 (4066055) | 4066055..4066540 | + | 486 | WP_000214393.1 | antirestriction protein | - |
| AOY60_RS19735 (4066556) | 4066556..4067032 | + | 477 | WP_001186774.1 | RadC family protein | - |
| AOY60_RS19740 (4067095) | 4067095..4067316 | + | 222 | WP_000692323.1 | DUF987 domain-containing protein | - |
| AOY60_RS19745 (4067396) | 4067396..4067773 | + | 378 | WP_001285620.1 | type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA | Antitoxin |
| AOY60_RS19750 (4067820) | 4067820..4068197 | + | 378 | WP_001094430.1 | TA system toxin CbtA family protein | Toxin |
| AOY60_RS19755 (4068194) | 4068194..4068682 | + | 489 | WP_000761666.1 | DUF5983 family protein | - |
| AOY60_RS19760 (4068694) | 4068694..4068891 | + | 198 | WP_001374283.1 | DUF957 domain-containing protein | - |
| AOY60_RS19765 (4068976) | 4068976..4069818 | + | 843 | WP_201473040.1 | DUF4942 domain-containing protein | - |
| AOY60_RS19770 (4070099) | 4070099..4070635 | - | 537 | WP_000942785.1 | GspM family type II secretion system protein YghD | - |
| AOY60_RS19775 (4070679) | 4070679..4072082 | - | 1404 | Protein_3866 | inorganic phosphate transporter PitB | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 13971.82 Da Isoelectric Point: 6.8608
>T103122 WP_001094430.1 NZ_CP028732:4067820-4068197 [Escherichia coli]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ
Download Length: 378 bp
>T103122 NZ_CP028732:4067820-4068197 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA
Antitoxin
Download Length: 126 a.a. Molecular weight: 13698.52 Da Isoelectric Point: 5.9505
>AT103122 WP_001285620.1 NZ_CP028732:4067396-4067773 [Escherichia coli]
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV
Download Length: 378 bp
>AT103122 NZ_CP028732:4067396-4067773 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y6M7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| PDB | 2INW | |
| AlphaFold DB | A0A0E0Y522 |